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keratinocyte growth medium ksfm  (PromoCell)


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    PromoCell keratinocyte growth medium ksfm
    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in <t>KSFM</t> medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).
    Keratinocyte Growth Medium Ksfm, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/keratinocyte+growth+medium+ksfm/Keratinocyte+Growth+Medium+2+Kit/pmc13123934-81-11-18
    Average 95 stars, based on 68 article reviews
    keratinocyte growth medium ksfm - by Bioz Stars, 2026-10
    95/100 stars

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    1) Product Images from "Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro"

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro

    Journal: PLOS One

    doi: 10.1371/journal.pone.0347228

    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).
    Figure Legend Snippet: PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Techniques Used: Expressing, Transfection, Control, Western Blot, Knockdown

    Related Articles

    Cell Culture:

    Article Title: Effects of miR-204-5p modulation on PAX6 regulation and corneal inflammation
    Article Snippet: .. Cells were then centrifuged at 200 g for 5 min and cultured in a Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Germany). .. T-LSC and mut-LSC cell lines were obtained as a gift from D. Aberdam, (INSERM, Paris) and have been described previously – .

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro
    Article Snippet: Trypsin was deactivated by adding Dulbecco’s Modified Eagle Medium (DMEM) (Cat. Nr. 11594426, ThermoFischer scientific, Inc.), containing 10% FCS. .. After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 . ..

    Article Title: Effects of miR-204-5p modulation on PAX6 regulation and corneal inflammation.
    Article Snippet: .. Cells were then centrifuged at 200 g for 5 min and cultured in a Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Germany). .. Telomerase-immortalized limbal stem cell (T-LSC), CRISPR/Cas9 mutated PAX6+/− LSC (mutLSC), and human corneal epithelial cell-transformed (HCE-T) cell lines T-LSC and mut-LSC cell lines were obtained as a gift from D. Aberdam, (INSERM, Paris) and have been described previously44–46.

    Centrifugation:

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro
    Article Snippet: Trypsin was deactivated by adding Dulbecco’s Modified Eagle Medium (DMEM) (Cat. Nr. 11594426, ThermoFischer scientific, Inc.), containing 10% FCS. .. After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 . ..



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    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in <t>KSFM</t> medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).
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    Image Search Results


    PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Journal: PLOS One

    Article Title: Fatty acid-binding protein 5 (FABP5) modulates limbal epithelial cell homeostasis by regulating the expression of key genes under both normal and inflammatory conditions , in vitro

    doi: 10.1371/journal.pone.0347228

    Figure Lengend Snippet: PAX6, TP53, TP63, FOSL2, FOXC1, PPARγ and NFκB mRNA expression (A) and PAX6 and FOSL2 protein levels (B) in LECs transfected with FABP5 siRNA or control (ctrl) siRNA. Western blot image from a single biological replicate (C) . Data are shown as mean ± SEM. Statistical analysis has been performed using two-way ANOVA followed by Tukey test (n = 7). p-values below 0.05 were considered statistically significant. PAX6 mRNA level was significantly lower in FABP5 knockdown LECs than in ctrl siRNA LECs, in KSFM medium or following LPS and IL-1β treatment (p ≤ 0.022). Furthermore, PAX6 protein level was significantly lower following FABP5 knockdown, than in ctrl siRNA LECs without treatment and after IL-1β treatment (p ≤ 0.031). TP63 mRNA level was significantly higher in ctrl siRNA LECs following LPS or IL-1β treatment, than without treatment (p ≤ 0.015). FOSL2 mRNA level was significantly higher in ctrl siRNA LECs following IL-1β treatment, than without treatment (p = 0.033). Under LPS and IL-1β treatment, FOSL2 mRNA levels were significantly lower in FABP5 knockdown cells than in control (transfected) cells (p ≤ 0.025).

    Article Snippet: After centrifugation at 200g for 5 minutes, cells were cultured in Keratinocyte growth medium (KSFM) (Cat. Nr. C-20111, Promocell, Heidelberg, Germany), at 37 oC, 5% CO 2 .

    Techniques: Expressing, Transfection, Control, Western Blot, Knockdown